EEffects of dietary lactobacillus on growth performance and intestinal health of Epinephelus fuscoguttatus♀× Epinephelus polyphekadion♂

In the report， in order to study the effects of dietary Lactobacillus on the growth performance， serum biochemical， activities of digestive enzymes and intestinal microflora of Epinephelus fuscoguttatus♀ × Epinephelus polyphekadion♂， 450 young fish with 89.55±0.75 g initial average body weight were randomly divided into five groups with three replicates per group. The fish in the control group （CG） were fed a basal diet， and that of the other experimental groups （EG） were fed the basal diet supplemented with 0.8% （EG Ⅰ）， 1.2% （EG Ⅱ）， 1.6% （EG Ⅲ）， and 2.0% （EG Ⅳ） of Lactobacillus plantarum and Lactobacillus acidophilus （2∶1） for eight weeks， and the growth characteristics， the biochemistry index of the serum， the activities of the digestive enzyme， and the gut flora were analyzed. The results indicated that （1） there are no significant differences between the weight gain rate （WGR）， the specific growth rate （SGR）， and the feed coefficient rate （FCR） of CG and that of EG groups. （2） the activity of ALT in EG II， the TP， ALT， ALT， TC and TG in EG III group， and AKP， ALT in EG Ⅳ were significantly higher than that of EG groups （P<0.05）. （3） with the increasing concentration of dietary Lactobacillus， the activity of intestinal AMS firstly increases， and then decrease， and there are no significant differences between the activities of the digestive enzyme of CG and that of EG groups. （4） there are no significant differences between the Ace， Chaol， Shannon and Simpson index of intestinal microflora of CG and that of EG groups， however， the relative abundance of Firmicutes of EG groups was significantly higher than that of CG group （P<0.05）. Our study suggested that there are some significant effects of L. plantarum and L. acidophilus on some serum biochemical indexes related to metabolism and intestinal digestive enzyme activity of hybrid grouper， and which can improve the intestinal microflora structure.