Abstract: Phosphorus-solubilizing Bacteria can decompose the insoluble phosphorus in the soil， which can effectively reduce the fixation of soil phosphorus and alleviate the problem of low phosphorus fertilizer utilization in the soil. In our report， the Halo zone on agar plate and the molybdenum antimony resistance colorimetric were used to obtain the high-efficiency solubilizing-phosphate bacteria strain. The morphological characteristics， the physiological and biochemical characteristics， the 16S rDNA gene sequence， were analyzed. The results showed that the bacteria strain is identified as Bacillus subtilis and named as GS032. The bacteria strain could secrete organic acids that cause the pH of the nutrient solution to drop significantly during culturing； the higher the initial inoculation amount， the higher the amount of soluble phosphorus contained in the final culture solution. When the initial culture medium pH was 5， the inoculation amount of bacteria was 8×107 cfu and the culture time was 3 days， the content of soluble phosphorus was the highest， which is 224 mg/L.