Abstract: In the report, solid phase extraction (SPE) and high performance liquid chromatography (HPLC) were performed to detect the occurrence and distribution of two tetracyclines and two macrolides in swine and chook feces sampled from large scale feeding operations in different areas of Hainan province. The results indicated that the average content and detection rate of tetracyclines compounds were higher than that of macrolides. The total concentrations of tetracyclines ranged from 0 μg·kg^-1 to 8 460.8 μg·kg^-1 (F. W.) with an average of 385.4 μg·kg^-1, the lowest detection rate was 53.2% in all swine dung samples. The dominant compounds in swine manures were oxytetracycline. The total concentrations of macrolides ranged from 0 μg·kg^-1 to 1 052.8 μg·kg^-1 (F. W.) with an average of 46.9 μg·kg^-1, the lowest detection rate was 25.9% in all swine dung samples. The dominant compound in swine manures was tylosin. The total concentrations of tetracyclines ranged from 0 μg·kg^-1 to 2 839.3 μg·kg^-1 (F. W.) with an average of 159.3 μg·kg^-1, the lowest detection rate was 57.1%. The dominant compound in chook manures was aureomycin. And macrolides ranged from 0 μg·kg^-1 to 845.2 μg·kg^-1 (F. W.) with an average of 28.1 μg·kg^-1, the lowest detection rate was 14.3% in all chook dung samples. The dominant compound in chook manures was kitasamycine. There were significant differences among the distribution characteristics of both compounds in swine and chook dungs, the amounts of two kinds of antibiotics in swine dungs from Qionghai and Lingao were higher than that of other places in Hainan, the amounts of two kinds of antibiotics in chook dungs from Danzhou and Qionghai were higher than that of other places in Hainan.