Abstract: In our report, a novel technology for LED backlight based on micro-dot structures mixed with quantum dots was studied, and a protocol was proposed, in which red and green quantum dots with a certain right proportion were filled into the micro-structure of light guide plate, in order to obtain white balance, blue LEDs were applied as the excitation light source. The key factors on the white balance of backlight were also studied, which includes concentration ratio of red/green quantum dots, light excitation intensity of blue LEDs, ink characteristics and so on. Based on the proposed method, a sample prototype of a backlight system was developed, the micro-dot structures mixed with R/G quantum dots of 625 nm/525 nm were excited by the blue LEDs of 450 nm. The test data indicated that the output color coordinates (CIE1931) of the sample was (0.3175, 0.314), color temperature was 6 281 K, and the color gamut coverage rate attained 119.3%. The results showed that the proposed method was feasible, and was a novel method of quantum dots based backlight, which was closer to the traditional manufacturing process of edge-lit backlight system.