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岩原鲤仔稚鱼生长发育过程中消化酶活性的变化

Fluctuations of Digestive Enzymes Activities in Rock Carp(Procypris rabaudi) During Early Ontogeny

  • 摘要: 本文分析了岩原鲤仔稚鱼的生长变化情况及其胰蛋白酶、脂肪酶、淀粉酶和碱性磷酸酶等消化酶的活性变化情况,探讨了饥饿对鱼体消化酶活性的影响.结果显示,岩原鲤仔稚鱼的阶段体重变化呈指数模式(y=2.749e0.0806x,R2=0.973),但它较其他大多数鱼类的生长缓慢(特定生长率为8.29%).在岩原鲤仔稚鱼开口前即能检测到胰蛋白酶、脂肪酶、淀粉酶和碱性磷酸酶的活性,其中,胰蛋白酶和脂肪酶的活性在岩原鲤生长发育前期(1~10 d)显著上升(P<0.05),而淀粉酶和碱性磷酸酶的活性在生长初期的变化不显著(P>0.05).本文测定的4种消化酶活性在岩原鲤仔稚鱼生长后期(25 d)均显著上升(P<0.05),并在30 d左右达到峰值,而后在40 d时显著下降(P<0.05).于饥饿状态下岩原鲤仔稚鱼在开口后7 d的成活率为0,胰蛋白酶和脂肪酶的活性受到显著影响(P<0.05),而淀粉酶和碱性磷酸酶的活性虽有所降低,但与投喂组相比其差异并不显著(P> 0.05).本实验结果表明,岩原鲤仔稚鱼在初期生长阶段需要含有充足蛋白质和脂肪的饵料或饲料,在其发育后期可以添加一定比例的碳水化合物,以作为其能量来源.

     

    Abstract: In the report, the growth performance and digestive enzymes activities during early ontogeny of rock carp, and the effects of starvation treatment on these parameters were analyzed. The results showed that the growth pattern of rock carp was exponential(y= 8.076 e0.0261x,R2 = 0.981), and the specific growth rate(SGR 8.29%) was much slower than most of the other fish species. The activities of trypsin, lipase, amylase and alkaline phosphatase could be detected before the mouth opening, and the activities of trypsin and lipase increased significantly during the early stage(1~10 d)(P<0.05). While the activities of amylase and alkaline phosphatase did not change significantly during the early growth period(P>0.05). The activities of these digestive enzymes showed a significant increased(P<0.05) during the later growth stage(25 d), and reached at peak in 30 days, and then significantly decreased afterwards(P<0.05). Under starvation condition, the survival rate was 0 on day 7 th after mouth opening, and the activity of protease and lipase were significantly affected by the(P<0.05). The activities of amylase and alkaline phosphatase were decreased, however, compared with feeding groups, the change were not significant(P>0.05). Our findings suggested that during the early growth stage, rock carp requires diets with sufficient protein and lipid, and the later growth stage of the fish larvae, a certain proportion of carbohydrate can be added as energy source.

     

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