Abstract: In our report, the literature research method, physical fitness test and mathematical statistics were performed to analyze the physical fitness feature of 634 students in Hainan University and compare the differences in terms of grade. The results indicated that the overall situation of Hainan University students' physique was the middle level (74.28 points); there was a significant difference among that of different gender, the score of girls (76.1 points) was better than that of boys (72.45 points); The total score of physical fitness of junior had the best test scores of physique best, 76.09 points, that of sophomores are 75.32 points, that of seniors was 71.72 points, moreover, the difference is significantly among the different grades. There is no obvious difference in the body shape of each grades, however, in the indexes of body function and physical quality, some of them have significant differences with statistical significance.