Abstract: In our report, in order to elucidate the effects of exogenous spermidine on the photosynthetic and physiological characteristics of native Camellia oleifera Abel in Hainan province under waterlogging stressed condition, and to evaluate the effects of exogenous Spd on improving the waterlogging resistance of C.oleifera, C.oleifera originating from Hainan province was used as a material. The results showed that waterlogging stress have significant negative impacts on stem diameter, Pn, Gs, Tr, Vpd, photosynthetic pigment content, leaf water potential, relative conductivity, and MDA content, exogenous spermidine application can alleviate efficiently negative effects caused by waterlogging stress on above parameters, among which the significantly effects on leaf water potential, relative conductivity, MDA content, and soluble protein can be found. Therefore, the photosynthetic characteristics and cell membrane system of C.oleifera can be efficiently improved by Spd application under waterlogging stressed condition. Our finding can also provide some scientific guidance to the cultivation of C.oleifera under extreme climatic conditions.