Abstract: In our report, the chemical synthesis method were performed to synthesis four antimicrobial peptides, temporin-GHa，temporin-GHb，temporin-GHc, and temporin-GHd, their antimicrobial activities were determined, and the effects of temperature, heat-treated time, and pH value on antimicrobial activities were analyzed. The results indicated that temporin-GHa～GHd has the antimicrobial activities against Gram-positive strains, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Gram-negative strains Escherichia coli (D 31), and fungus, Monilia albican; S. aureus was the most sensitive strains, and the minimum inhibitory concentration (MIC) of four temporin family antimicrobial peptides against S. aureus is 6.8, 6.8, 12.9, and 12.7 μM, respectively; temporin GHa，temporin GHc和temporin GHd were stable over a broad range of temperatures (25-100 ℃), and pH (2-10).